Effect of cytokines and ultraviolet B radiation on the promoter activity of the metallothionein gene in keratinocytes

Narumi, Hiromi and Nakano, Hajime and Kaneko, Takahide and Nakajima, Koji and Matsuzaki, Yasushi and Aizu, Takayuki and Sawamura, Daisuke and Hanada, Katsumi (2013) Effect of cytokines and ultraviolet B radiation on the promoter activity of the metallothionein gene in keratinocytes. Advances in Bioscience and Biotechnology, 04 (09). pp. 896-899. ISSN 2156-8456

[thumbnail of ABB_2013090316365953.pdf] Text
ABB_2013090316365953.pdf - Published Version

Download (308kB)

Abstract

Metallothionein (MT) has many functions that are modulated by several factors, including ultraviolet (UV) radiation and cytokines. We thought that these diverse functions of MT might reflect the specific regulatory mechanisms of its expression. To understand some of the molecular mechanisms underlying MT expression, we examined the effects of several cytokines and UVB on the promoter activity of the MT gene. First, we introduced the MT promoter construct into the HaCaT keratinocytes and treated them with various concentrations of interleukin-1α (IL-1α) and IL-6. The addition of IL-1α and IL-6 led to an increase in the promoter activity of the MT gene. UVB is known to induce MT expression in epidermal keratinocytes, and IL-6 is a possible mediator of MT induction by UV radiation. Therefore, we investigated whether UVB could induce MT promoter activity. Our results showed, interestingly, that UVB radiation has no or little effect on the promoter activity. This suggested a complex molecular regulation of the MT gene.

Item Type: Article
Subjects: GO for STM > Biological Science
Depositing User: Unnamed user with email support@goforstm.com
Date Deposited: 18 Mar 2023 09:07
Last Modified: 31 Aug 2023 04:39
URI: http://archive.article4submit.com/id/eprint/360

Actions (login required)

View Item
View Item