Molecular Characterization of Ralstonia mannitolilytica Isolated from the Gut of Brahmina coriacea Grubs Using 16S Ribosomal RNA Gene Sequence Analysis

Ralstonia mannitolilytica is an emerging aerobic gram-negative bacterium causing infection among immune compromised patients. It has been isolated from the soil, water, human body and plant surfaces but this is the first report of Ralstonia mannitolilytica isolated from the grubs of Brahmina coriacea in the world. The gut microbiota was isolated from different ecotypes of B. coriacea, collected from various parts of Himachal Pradesh, India. Ralstonia mannitolilytica strain GMG5 was found from the grubs of Seobagh population. Ralstonia mannitolilytica was able to degrade cellulose in the Carboxy Methyl Cellulose (CMC) media, where the cellulolytic index was recorded to be 0.33. The Cellulolytic bacterial isolates were identified using morphological, biochemical and 16S ribosomal RNA gene sequence analysis.