Chemical Characterization of Supercritical Fluid Extract (SFE) of Ailanthus excelsa and Estimation of Total Phenols and Flavonoids in SFE

Ailanthus excelsa which is usually called as tree of heaven is known to possess anti- fungal activity. The bio constituents such as flavonoids and phenols which are present in the leaves of Ailanthus excelsa is known to possess anti- bacterial, anti- fungal properties. This experiment was conducted to study the fungicidal activity of Ailanthus excelsa and its characeterization. Results revealed that the supercritical fluid extraction was effective at 60⁰C of temperature and 225 bar pressure with solvent flow rate of 5 g/min and caron di- oxide flow rate at 20 g/min. The GC-MS analysis showed that forty different constituents were present in the A. excelsa. Among which, Ricinoleic acid, Diethyl phthalate, 1.63 Hexadecanoic acid, methyl ester and 9- octadecenoic acid (Z)-, methyl ester were found to impart anti- fungal activity. The total phenols and flavonoids was 19.65 mgGAEg-1 and 40.68 mgGAEg-1 respectively was found in the SFE extract of A. excelsa.