PHYTOHORMONAL EFFECT ON in vitro CALLUS INDUCTION OF Tinospora cordifolia (Willd.) Miers.

Tinospora cordifolia is a medicinally important plant and has special place in the Indian therapies of disease treatments hence listed in a prioritized medicinal plant. Because of modern day deforestation and over harvesting of medicinal plants, we need alternate rapid regeneration methods for such medicinally important plants as their seeds are not easily available and dramatic change in the environment because high dose of pollution not easily favors the growth of seedlings. The in vitro regeneration technique provide efficient tool for multiplication of plants on large scale. Hence, in this study we attempt to induce callus formation and regenerations of shoot by using leaf bit and shoot tip in MS media amended with different combinations of phyto-hormones. We observed that the combinations of BAP with 2, 4 D shown significant induction of callus with maximum average fresh weight while regeneration of shoot not observed in any combinations of phyto-hormones.