THERAPEUTIC POTENTIALS OF BIOSYNTHESIZED SILVER NANOPARTICLES FROM Phyllanthus amarus LEAF AGAINST RESISTANT CLINICAL BACTERIAL ISOLATES

The endurance of infectious agents to medications is a serious danger worldwide. This endurance to medications implies increase in number of death that can leads to decrease in human population. Antibiotic resistance mostly triggered by antibiotic resistance gene (ARG) is the most terrifying. The present therapeutic measures used on antibiotic enduring bacteria have a setback like its inability to kill the target bacteria. Both the search and interest for brand-new therapeutics that can overpower this setback has preceded to massive investigation on plants phytochemicals. Preparations from plants sources tend to be favourable with little or no side effects in comparison with synthetic drugs. Nevertheless curative attribute of preparations from plant sources becomes conceded as a result of their little bio-obtainability. Incorporation of the plants phytochemicals with innovative methods of delivery in form of nanocarriers can overpower this setback. The aim of the research is to verify the therapeutic and ethnopharmacological potentials of bio-synthesized AgNPs from Phyllanthus amarus plant leaf as an antibacterial agent against resistant bacterial isolates. Preliminary phytochemical analysis showed the presence of flavonoids, saponins, tannins, glycosides and triterpenoids. The in vitro antibacterial activity of biologically synthesized AgNPs from Phyllanthus amarus aqueous leaf extract was determined by agar disc diffusion and broth dilution methods. The results of the bioassay obtained showed that the biologically synthesized AgNPs had antibacterial potential against the clinical resistance bacterial isolates. Both the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) for the biologically synthesized AgNPs ranges from 125µg/ml-500 µg/ml.