Medeiros, Liciane F. and Battastini, Ana Maria O. and Souza, Andressa de and Rozisky, Joanna R. and Santos, Vinicius S. dos and Caumo, Wolnei and Torres, Iraci L.S. (2014) One-time Exposure to General Anesthetics Alters Nociceptive Response and Nucleotide Hydrolysis in Infant Rats. British Journal of Medicine and Medical Research, 4 (22). pp. 3975-3989. ISSN 22310614
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Abstract
Aims: The objective was to evaluate the single exposure of general anesthetics with or without a surgical procedure at postnatal day 14 (P14) on nociceptive behavioral responses. Furthermore, we evaluated ectonucleotidase activities at P14 and P30.
Place of Study: All experiments were performed at the Animal Experimentation Unit of Hospital de Clínicas de Porto Alegre. The Institutional Committee approved the experimental protocol f (GPPG-HCPA protocol No: 08149).
Methodology: Fourteen-day-old male Wistar rats were divided into two experimental designs (ED): the 1stED – control (C), isoflurane (ISO), isoflurane/surgery (ISO-SUR) and the 2nd ED – control (C), fentanyl/S(+)-ketamine (FK) and fentanyl/S(+)-ketamine/surgery (FK-SUR). Nociceptive responses were evaluated using the formalin and tail-flick tests, and the ectonucleotidase activities were evaluated by spinal cord synaptosome. All assessments were performed at P14 and P30.
Results: The FK and FK-SUR groups displayed an increased latency at P30. For the ectonucleotidase activity analysis, the following results were observed: (a) in the 1st ED, the ISO group displayed a reduction in ATPase and ADPase, and both ISO and ISO-SUR displayed a reduction in AMPase activity at P14; (b) in the 2nd ED, the FK group displayed an increase in AMPase activity at P14 and increased ATPase activity at P30, and both FK and FK-SUR exhibited an increase in AMPase activity at P30.
Conclusion: Our results indicate that single administration of general anesthetics at P14 is able to promote changes in the nociceptive response in the intermediate-term, and in the ectonucleotidase activities in the short- and medium-terms.
Item Type: | Article |
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Subjects: | GO for STM > Medical Science |
Depositing User: | Unnamed user with email support@goforstm.com |
Date Deposited: | 11 Jul 2023 04:40 |
Last Modified: | 16 Jan 2024 04:32 |
URI: | http://archive.article4submit.com/id/eprint/1088 |