One-time Exposure to General Anesthetics Alters Nociceptive Response and Nucleotide Hydrolysis in Infant Rats

Medeiros, Liciane F. and Battastini, Ana Maria O. and Souza, Andressa de and Rozisky, Joanna R. and Santos, Vinicius S. dos and Caumo, Wolnei and Torres, Iraci L.S. (2014) One-time Exposure to General Anesthetics Alters Nociceptive Response and Nucleotide Hydrolysis in Infant Rats. British Journal of Medicine and Medical Research, 4 (22). pp. 3975-3989. ISSN 22310614

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Abstract

Aims: The objective was to evaluate the single exposure of general anesthetics with or without a surgical procedure at postnatal day 14 (P14) on nociceptive behavioral responses. Furthermore, we evaluated ectonucleotidase activities at P14 and P30.
Place of Study: All experiments were performed at the Animal Experimentation Unit of Hospital de Clínicas de Porto Alegre. The Institutional Committee approved the experimental protocol f (GPPG-HCPA protocol No: 08149).
Methodology: Fourteen-day-old male Wistar rats were divided into two experimental designs (ED): the 1stED – control (C), isoflurane (ISO), isoflurane/surgery (ISO-SUR) and the 2nd ED – control (C), fentanyl/S(+)-ketamine (FK) and fentanyl/S(+)-ketamine/surgery (FK-SUR). Nociceptive responses were evaluated using the formalin and tail-flick tests, and the ectonucleotidase activities were evaluated by spinal cord synaptosome. All assessments were performed at P14 and P30.
Results: The FK and FK-SUR groups displayed an increased latency at P30. For the ectonucleotidase activity analysis, the following results were observed: (a) in the 1st ED, the ISO group displayed a reduction in ATPase and ADPase, and both ISO and ISO-SUR displayed a reduction in AMPase activity at P14; (b) in the 2nd ED, the FK group displayed an increase in AMPase activity at P14 and increased ATPase activity at P30, and both FK and FK-SUR exhibited an increase in AMPase activity at P30.
Conclusion: Our results indicate that single administration of general anesthetics at P14 is able to promote changes in the nociceptive response in the intermediate-term, and in the ectonucleotidase activities in the short- and medium-terms.

Item Type: Article
Subjects: GO for STM > Medical Science
Depositing User: Unnamed user with email support@goforstm.com
Date Deposited: 11 Jul 2023 04:40
Last Modified: 16 Jan 2024 04:32
URI: http://archive.article4submit.com/id/eprint/1088

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