Adding function to the genome of African Salmonella Typhimurium ST313 strain D23580

Canals, Rocío and Hammarlöf, Disa L. and Kröger, Carsten and Owen, Siân V. and Fong, Wai Yee and Lacharme-Lora, Lizeth and Zhu, Xiaojun and Wenner, Nicolas and Carden, Sarah E. and Honeycutt, Jared and Monack, Denise M. and Kingsley, Robert A. and Brownridge, Philip and Chaudhuri, Roy R. and Rowe, Will P. M. and Predeus, Alexander V. and Hokamp, Karsten and Gordon, Melita A. and Hinton, Jay C. D. and Waldor, Matthew K. (2019) Adding function to the genome of African Salmonella Typhimurium ST313 strain D23580. PLOS Biology, 17 (1). e3000059. ISSN 1545-7885

[thumbnail of file_id=10.1371%2Fjournal.pbio.3000059&type=printable] Text
file_id=10.1371%2Fjournal.pbio.3000059&type=printable - Published Version

Download (4MB)

Abstract

Salmonella Typhimurium sequence type (ST) 313 causes invasive nontyphoidal Salmonella (iNTS) disease in sub-Saharan Africa, targeting susceptible HIV+, malarial, or malnourished individuals. An in-depth genomic comparison between the ST313 isolate D23580 and the well-characterized ST19 isolate 4/74 that causes gastroenteritis across the globe revealed extensive synteny. To understand how the 856 nucleotide variations generated phenotypic differences, we devised a large-scale experimental approach that involved the global gene expression analysis of strains D23580 and 4/74 grown in 16 infection-relevant growth conditions. Comparison of transcriptional patterns identified virulence and metabolic genes that were differentially expressed between D23580 versus 4/74, many of which were validated by proteomics. We also uncovered the S. Typhimurium D23580 and 4/74 genes that showed expression differences during infection of murine macrophages. Our comparative transcriptomic data are presented in a new enhanced version of the Salmonella expression compendium, SalComD23580: http://bioinf.gen.tcd.ie/cgi-bin/salcom_v2.pl. We discovered that the ablation of melibiose utilization was caused by three independent SNP mutations in D23580 that are shared across ST313 lineage 2, suggesting that the ability to catabolize this carbon source has been negatively selected during ST313 evolution. The data revealed a novel, to our knowledge, plasmid maintenance system involving a plasmid-encoded CysS cysteinyl-tRNA synthetase, highlighting the power of large-scale comparative multicondition analyses to pinpoint key phenotypic differences between bacterial pathovariants.

Item Type: Article
Subjects: GO for STM > Biological Science
Depositing User: Unnamed user with email support@goforstm.com
Date Deposited: 05 Jan 2023 10:22
Last Modified: 21 Aug 2023 11:24
URI: http://archive.article4submit.com/id/eprint/7

Actions (login required)

View Item
View Item