In vitro CALLUS INDUCTION AND BULBLET REGENERATION OF HYACINTH (Hyacinthus orientalis L.)

The influence of explants type and growth regulators on in vitro callus induction and bulblet formation was studied in hyacinth (Hyacinthus orientalis L.) cultivars of Pink Pearl and Blue Jacket. For callus induction, bulb scales and leaves were cultured on Murashige and Skoog (MS) media containing 0.3 mg L-1 IBA (3-indolebutyric acid), and different concentrations of 6-benzylaminopurine (BAP) (0.5, 1 and 2 mg L-1) and kinetin (Kin) (0.5 and 1 mg L-1) in combination. Callus induction was successfully obtained on MS media supplemented with a combination of two cytokinins compared to BAP and Kin alone. The combination of 1 mg L-1 BAP and 1 mg L-1 Kin from scale and 2 mg L-1 BAP and 1 mg L-1Kin in both explants were observed the best in term of callus fresh weight for cultivars Pink Pearl and Blue Jacket, respectively. For direct and indirect bulblets regeneration, bulb scales, leaves and calli were subcultured on MS medium containing 0, 0.5, 1 and 1.5 mg L-1 IBA and NAA in combination. The mean fresh weight of direct and indirect regenerated bulblet and resultant calli were stimulated to form bulblets by increasing the BA concentration. Bulblets were not produced in the presence of NAA alone. Bulblets developed roots well on MS media supplemented with1 mg L-1NAA in both cultivars.